A whole-genome RNAi Screen for C. elegans miRNA pathway genes.

ABSTRACT:

Background

miRNAs are an abundant class of small, endogenous regulatory RNAs. Although it is now appreciated that miRNAs are involved in a broad range of biological processes, relatively little is known about the actual mechanism by which miRNAs downregulate target gene expression. An exploration of which protein cofactors are necessary for a miRNA to downregulate a target gene should reveal more fully the molecular mechanisms by which miRNAs are processed, trafficked, and regulate their target genes.

Results

A weak allele of the C. elegans miRNA gene let-7 was used as a sensitized genetic background for a whole-genome RNAi screen to detect miRNA pathway genes, and 213 candidate miRNA pathway genes were identified. About 2/3 of the 61 candidates with the strongest phenotype

SUBMITTER: Parry DH 

PROVIDER: S-EPMC2211719 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature