Establishment of mouse embryonic stem cell-derived erythroid progenitor cell lines able to produce functional red blood cells.

ABSTRACT:

Background

The supply of transfusable red blood cells (RBCs) is not sufficient in many countries. If erythroid cell lines able to produce transfusable RBCs in vitro were established, they would be valuable resources. However, such cell lines have not been established. To evaluate the feasibility of establishing useful erythroid cell lines, we attempted to establish such cell lines from mouse embryonic stem (ES) cells.

Methodology/principal findings

We developed a robust method to obtain differentiated cell lines following the induction of hematopoietic differentiation of mouse ES cells and established five independent hematopoietic cell lines using the method. Three of these lines exhibited characteristics of erythroid cells. Although their precise characteristics varied, ea

SUBMITTER: Hiroyama T 

PROVIDER: S-EPMC2212133 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature