Passage and reversal effects on gene expression of bovine meniscal fibrochondrocytes.

ABSTRACT: The knee meniscus contains a mixed population of cells that exhibit fibroblastic as well as chondrocytic characteristics. Tissue engineering studies and future therapies for the meniscus require a large population of cells that are seeded on scaffolds. To achieve this, monolayer expansion is often used as a technique to increase cell number. However, the phenotype of these cells may be significantly different from that of the primary population. The objective of this study was to investigate changes in meniscal fibrochondrocytes at the gene expression level over four passages using quantitative real-time reverse transcriptase polymerase chain reaction. Cells from the inner two-thirds of bovine medial menisci were used. Four extracellular matrix (ECM) molecules, commonly found in the menisc

SUBMITTER: Gunja NJ 

PROVIDER: S-EPMC2212577 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature