Asymmetry of the GroEL-GroES complex under physiological conditions as revealed by small-angle x-ray scattering.

ABSTRACT: Despite the well-known functional importance of GroEL-GroES complex formation during the chaperonin cycle, the stoichiometry of the complex has not been clarified. The complex can occur either as an asymmetric 1:1 GroEL-GroES complex or as a symmetric 1:2 GroEL-GroES complex, although it remains uncertain which type is predominant under physiological conditions. To resolve this question, we studied the structure of the GroEL-GroES complex under physiological conditions by small-angle x-ray scattering, which is a powerful technique to directly observe the structure of the protein complex in solution. We evaluated molecular structural parameters, the radius of gyration and the maximum dimension of the complex, from the x-ray scattering patterns under various nucleotide conditions (3 mM ADP,

SUBMITTER: Inobe T 

PROVIDER: S-EPMC2212710 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature