Fine mapping of posttranslational modifications of the linker histone H1 from Drosophila melanogaster.

ABSTRACT: The linker histone H1 binds to the DNA in between adjacent nucleosomes and contributes to chromatin organization and transcriptional control. It is known that H1 carries diverse posttranslational modifications (PTMs), including phosphorylation, lysine methylation and ADP-ribosylation. Their biological functions, however, remain largely unclear. This is in part due to the fact that most of the studies have been performed in organisms that have several H1 variants, which complicates the analyses. We have chosen Drosophila melanogaster, a model organism, which has a single H1 variant, to approach the study of the role of H1 PTMs during embryonic development. Mass spectrometry mapping of the entire sequence of the protein showed phosphorylation only in the ten N-terminal amino acids, mostly at

SUBMITTER: Villar-Garea A 

PROVIDER: S-EPMC2212714 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature