Systematic validation and atomic force microscopy of non-covalent short oligonucleotide barcode microarrays.

ABSTRACT:

Background

Molecular barcode arrays provide a powerful means to analyze cellular phenotypes in parallel through detection of short (20-60 base) unique sequence tags, or "barcodes", associated with each strain or clone in a collection. However, costs of current methods for microarray construction, whether by in situ oligonucleotide synthesis or ex situ coupling of modified oligonucleotides to the slide surface are often prohibitive to large-scale analyses.

Methodology/principal findings

Here we demonstrate that unmodified 20mer oligonucleotide probes printed on conventional surfaces show comparable hybridization signals to covalently linked 5'-amino-modified probes. As a test case, we undertook systematic cell size analysis of the budding yeast Saccharomyces cerevisiae genome

SUBMITTER: Cook MA 

PROVIDER: S-EPMC2212718 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature