Impact of DNA ligase IV on nonhomologous end joining pathways during class switch recombination in human cells.

ABSTRACT: Class switch recombination (CSR) is a region-specific, transcriptionally regulated, nonhomologous recombinational process that is initiated by activation-induced cytidine deaminase (AID). The initial lesions in the switch (S) regions are subsequently processed and resolved, leading to recombination of the two targeted S regions. The mechanisms by which repair and ligation of the broken DNA ends occurs is still elusive. Recently, a small number of patients lacking DNA ligase IV, a critical component of the nonhomologous end joining (NHEJ) machinery, have been identified. We show that these patients display a considerably increased donor/acceptor homology at Smu-Salpha junctions compared with healthy controls. In contrast, Smu-Sgamma junctions show an increased frequency of insertions but no

SUBMITTER: Pan-Hammarstrom Q 

PROVIDER: S-EPMC2212791 | biostudies-literature | 2005 Jan

REPOSITORIES: biostudies-literature