Cell adaptive response to extracellular matrix density is controlled by ICAP-1-dependent beta1-integrin affinity.

ABSTRACT: Cell migration is an integrated process requiring the continuous coordinated assembly and disassembly of adhesion structures. How cells orchestrate adhesion turnover is only partially understood. We provide evidence for a novel mechanistic insight into focal adhesion (FA) dynamics by demonstrating that integrin cytoplasmic domain-associated protein 1 (ICAP-1) slows down FA assembly. Live cell imaging, which was performed in both Icap-1-deficient mouse embryonic fibroblasts and cells expressing active beta(1) integrin, shows that the integrin high affinity state favored by talin is antagonistically controlled by ICAP-1. This affinity switch results in modulation in the speed of FA assembly and, consequently, of cell spreading and migration. Unexpectedly, the ICAP-1-dependent decrease in int

SUBMITTER: Millon-Fremillon A 

PROVIDER: S-EPMC2213582 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature