The full-ORF clone resource of the German cDNA Consortium.

ABSTRACT:

Background

With the completion of the human genome sequence the functional analysis and characterization of the encoded proteins has become the next urging challenge in the post-genome era. The lack of comprehensive ORFeome resources has thus far hampered systematic applications by protein gain-of-function analysis. Gene and ORF coverage with full-length ORF clones thus needs to be extended. In combination with a unique and versatile cloning system, these will provide the tools for genome-wide systematic functional analyses, to achieve a deeper insight into complex biological processes.

Results

Here we describe the generation of a full-ORF clone resource of human genes applying the Gateway cloning technology (Invitrogen). A pipeline for efficient cloning and sequencing was d

SUBMITTER: Bechtel S 

PROVIDER: S-EPMC2213676 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature