Dynein regulates epithelial polarity and the apical localization of stardust A mRNA.

ABSTRACT: Intense investigation has identified an elaborate protein network controlling epithelial polarity. Although precise subcellular targeting of apical and basolateral determinants is required for epithelial architecture, little is known about how the individual determinant proteins become localized within the cell. Through a genetic screen for epithelial defects in the Drosophila follicle cells, we have found that the cytoplasmic Dynein motor is an essential regulator of apico-basal polarity. Our data suggest that Dynein acts through the cytoplasmic scaffolding protein Stardust (Sdt) to localize the transmembrane protein Crumbs, in part through the apical targeting of specific sdt mRNA isoforms. We have mapped the sdt mRNA localization signal to an alternatively spliced coding exon. Intriguin

SUBMITTER: Horne-Badovinac S 

PROVIDER: S-EPMC2213700 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature