Plasticity of the systemic inflammatory response to acute infection during critical illness: development of the riboleukogram.

ABSTRACT:

Background

Diagnosis of acute infection in the critically ill remains a challenge. We hypothesized that circulating leukocyte transcriptional profiles can be used to monitor the host response to and recovery from infection complicating critical illness.

Methodology/principal findings

A translational research approach was employed. Fifteen mice underwent intratracheal injections of live P. aeruginosa, P. aeruginosa endotoxin, live S. pneumoniae, or normal saline. At 24 hours after injury, GeneChip microarray analysis of circulating buffy coat RNA identified 219 genes that distinguished between the pulmonary insults and differences in 7-day mortality. Similarly, buffy coat microarray expression profiles were generated from 27 mechanically ventilated patients every two days for

SUBMITTER: McDunn JE 

PROVIDER: S-EPMC2215774 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature