The oxidative stress adaptor p66Shc is required for permanent embryo arrest in vitro.

ABSTRACT:

Background

Excessive developmental failure occurs during the first week of in vitro embryo development due to elevated levels of cell death and arrest. We hypothesize that permanently arrested embryos enter a stress-induced "senescence-like" state that is dependent on the oxidative stress-adaptor and lifespan determinant protein p66Shc. The aim of this study was to selectively diminish p66Shc gene expression in bovine oocytes and embryos using post-transcriptional gene silencing by RNA-mediated interference to study the effects of p66Shc knockdown on in vitro fertilized bovine embryos.

Results

Approximately 12,000-24,000 short hairpin (sh)RNAi molecules specific for p66Shc were microinjected into bovine germinal vesicle stage oocytes or zygotes. Experiments were comprised of

SUBMITTER: Favetta LA 

PROVIDER: S-EPMC2220003 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature