Proteomic analysis of the amyloid precursor protein fragment C99: expression in yeast.

ABSTRACT: The accumulation and aggregation of fragments of amyloid precursor protein (APP) are central to the development of Alzheimer's disease. The production of the small fragment C99 is thought to form the rate-limiting step in the APP processing pathway, which can lead to the production of the toxic Abeta peptide. It has also been suggested that the proteasome contributes to APP catabolism. While the identities and aggregation propensities of many APP fragments have been studied in vitro, the sequences, structures, and cellular sources of fragments generated in vivo remains poorly elucidated. To better identify the specific APP fragments generated in vivo and to elucidate the role of the proteasome in APP processing, we developed a C99 yeast expression system. Using Zip Tip immunocapture, a spe

SUBMITTER: Sparvero LJ 

PROVIDER: S-EPMC2220045 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature