Aberrant overexpression of an epithelial marker, 14-3-3sigma, in a subset of hematological malignancies.

ABSTRACT:

Background

14-3-3sigma is a p53-mediated cell-cycle inhibitor in epithelial cells. The expression of 14-3-3sigma is frequently altered in cancers of epithelial origin associated with altered DNA methylation. Since its involvement in a non-epithelial tumor is unknown, we examined 14-3-3sigma expression in patients with haematological malignancies.

Methods

We analyzed 41 hematopoietic cell lines and 129 patients with a variety of hematological malignancies for 14-3-3sigma expression with real-time RT-PCR. We also examined protein levels by Western blot analysis and DNA methylation status of the 14-3-3sigma gene by methylation-specific PCR analysis of bisulfite-treated DNA. In addition, mutations of p53 gene were identified by RT-PCR-SSCP analysis and the expression levels of 1

SUBMITTER: Motokura T 

PROVIDER: S-EPMC2222637 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature