Analysis of the human kinome using methods including fold recognition reveals two novel kinases.

ABSTRACT:

Background

Protein sequence similarity is a commonly used criterion for inferring the unknown function of a protein from a protein of known function. However, proteins can diverge significantly over time such that sequence similarity is difficult, if not impossible, to find. In some cases, a structural similarity remains over long evolutionary time scales and once detected can be used to predict function.

Methodology/principal findings

Here we employed a high-throughput approach to assign structural and functional annotation to the human proteome, focusing on the collection of human protein kinases, the human kinome. We compared human protein sequences to a library of domains from known structures using WU-BLAST, PSI-BLAST, and 123D. This approach utilized both sequence comp

SUBMITTER: Briedis KM 

PROVIDER: S-EPMC2223070 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature