Transcription-associated recombination is dependent on replication in Mammalian cells.

ABSTRACT: Transcription can enhance recombination; this is a ubiquitous phenomenon from prokaryotes to higher eukaryotes. However, the mechanism of transcription-associated recombination in mammalian cells is poorly understood. Here we have developed a construct with a recombination substrate in which levels of recombination can be studied in the presence or absence of transcription. We observed a direct enhancement in recombination when transcription levels through the substrate were increased. This increase in homologous recombination following transcription is locus specific, since homologous recombination at the unrelated hprt gene is unaffected. In addition, we have shown that transcription-associated recombination involves both short-tract and long-tract gene conversions in mammalian cells, wh

SUBMITTER: Gottipati P 

PROVIDER: S-EPMC2223284 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature