Elucidating the function of an ancient NF-kappaB p100 homologue, CrRelish, in antibacterial defense.

ABSTRACT: The family of NF-kappaB transcription factors essentially regulates immune-related gene expression. Recently, we isolated and characterized the classical NF-kappaB/inhibitor kappaB (IkappaB) homologues from a "living fossil," the horseshoe crab, Carcinoscorpius rotundicauda. Interestingly, this ancient species also harbors another class I NF-kappaB p100 homologue, C. rotundicauda Relish (CrRelish). Similar to Drosophila Relish and the mammalian p100, CrRelish contains both the Rel-homology domains (RHD) and the IkappaB-like domain. In this study, we found that the RHD of CrRelish can recognize horseshoe crab and human kappaB response elements and activate the downstream reporter in vitro, thereby suggesting the evolutionary conservation of this molecule. Pseudomonas aeruginosa infection tr

SUBMITTER: Fan ZH 

PROVIDER: S-EPMC2223444 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature