Profiling bovine antibody responses to Mycobacterium avium subsp. paratuberculosis infection by using protein arrays.

ABSTRACT: With the genome sequence of Mycobacterium avium subsp. paratuberculosis determined, technologies are now being developed for construction of protein arrays to detect the presence of antibodies against M. avium subsp. paratuberculosis in host serum. The power of this approach is that it enables a direct comparison of M. avium subsp. paratuberculosis proteins to each other in relation to their immunostimulatory capabilities. In this study, 93 recombinant proteins, produced in Escherichia coli, were arrayed and spotted onto nitrocellulose. These proteins include unknown hypothetical proteins and cell surface proteins as well as proteins encoded by large sequence polymorphisms present uniquely in M. avium subsp. paratuberculosis. Also included were previously reported or known M. avium subsp.

SUBMITTER: Bannantine JP 

PROVIDER: S-EPMC2223459 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature