CodY of Streptococcus pneumoniae: link between nutritional gene regulation and colonization.

ABSTRACT: CodY is a nutritional regulator mainly involved in amino acid metabolism. It has been extensively studied in Bacillus subtilis and Lactococcus lactis. We investigated the role of CodY in gene regulation and virulence of the human pathogen Streptococcus pneumoniae. We constructed a codY mutant and examined the effect on gene and protein expression by microarray and two-dimensional differential gel electrophoresis analysis. The pneumococcal CodY regulon was found to consist predominantly of genes involved in amino acid metabolism but also several other cellular processes, such as carbon metabolism and iron uptake. By means of electrophoretic mobility shift assays and DNA footprinting, we showed that most of the targets identified are under the direct control of CodY. By mutating DNA predicte

SUBMITTER: Hendriksen WT 

PROVIDER: S-EPMC2223708 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature