Visualization of AP-1 NF-kappaB ternary complexes in living cells by using a BiFC-based FRET.

ABSTRACT: Protein-protein interactions are essential for maintaining cell structure and for executing almost all cellular processes. Determination of where and how each protein interacts with its partners provides significant insight into proteins' cellular roles. Although several assays, such as FRET and bimolecular fluorescence complementation (BiFC), have been developed and widely used for visualization and identification of protein interactions in living cells, there is no simple and convenient assay to visualize and identify multiple protein complexes in living cells. Because many signaling molecules often function as ternary complexes, availability of an assay for visualization and identification of ternary complexes will significantly expand the repertoire of protein interaction studies in li

SUBMITTER: Shyu YJ 

PROVIDER: S-EPMC2224177 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature