Prospective identification of enteroviruses involved in meningitis in 2006 through direct genotyping in cerebrospinal fluid.

ABSTRACT: Enterovirus infections were investigated with special emphasis on performing rapid molecular identification of enterovirus serotypes responsible for aseptic meningitis directly in cerebrospinal fluid (CSF). Enterovirus genotyping was carried out directly with specimens tested for the diagnostic procedure, using two seminested PCR assays designed to amplify the complete and partial gene sequences encoding the VP1 and VP4/VP2 capsid proteins, respectively. The method was used for identifying the enterovirus serotypes involved in meningitis in 45 patients admitted in 2005. Enterovirus genotyping was achieved in 98% of the patients studied, and we obtained evidence of 10 of the most frequent serotypes identified earlier by genotyping of virus isolates. The method was applied for the prospectiv

SUBMITTER: Mirand A 

PROVIDER: S-EPMC2224282 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature