Real-time PCR assays of single-nucleotide polymorphisms defining the major Brucella clades.

ABSTRACT: Members of the genus Brucella are known worldwide as pathogens of wildlife and livestock and are the most common organisms of zoonotic infection in humans. In general, brucellae exhibit a range of host specificity in animals that has led to the identification of at least seven Brucella species. The genomes of the various Brucella species are highly conserved, which makes the differentiation of species highly challenging. However, we found single-nucleotide polymorphisms (SNPs) in housekeeping and other genes that differentiated the seven main Brucella species or clades and thus enabled us to develop real-time PCR assays based around these SNPs. Screening of a diverse panel of 338 diverse isolates with these assays correctly identified each isolate with its previously determined Brucella cl

SUBMITTER: Foster JT 

PROVIDER: S-EPMC2224295 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature