Purification, crystallization and preliminary X-ray study of the fungal laccase from Cerrena maxima.

ABSTRACT: Laccases are members of the blue multi-copper oxidase family that oxidize substrate molecules by accepting electrons at a mononuclear copper centre and transferring them to a trinuclear centre. Dioxygen binds to the trinuclear centre and, following the transfer of four electrons, is reduced to two molecules of water. Crystals of the laccase from Cerrena maxima have been obtained and X-ray data were collected to 1.9 A resolution using synchrotron radiation. A preliminary analysis shows that the enzyme has the typical laccase structure and several carbohydrate sites have been identified. The carbohydrate chains appear to be involved in stabilization of the intermolecular contacts in the crystal structure, thus promoting the formation of well ordered crystals of the enzyme. Here, the results

SUBMITTER: Lyashenko AV 

PROVIDER: S-EPMC2225196 | biostudies-literature | 2006 Oct

REPOSITORIES: biostudies-literature