An accurate method for quantifying and analyzing copy number variation in porcine KIT by an oligonucleotide ligation assay.

ABSTRACT:

Background

Aside from single nucleotide polymorphisms, copy number variations (CNVs) are the most important factors in susceptibility to genetic disorders because they affect expression levels of genes. In previous studies, pyrosequencing, mini-sequencing, real-time PCR, invader assays and other techniques have been used to detect CNVs. However, the higher the copy number in a genome, the more difficult it is to resolve the copies, so a more accurate method for measuring CNVs and assigning genotype is needed.

Results

PCR followed by a quantitative oligonucleotide ligation assay (qOLA) was developed for quantifying CNVs. The accuracy and precision of the assay were evaluated for porcine KIT, which was selected as a model locus. Overall, the root mean squares of bias and stand

SUBMITTER: Seo BY 

PROVIDER: S-EPMC2228321 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature