Computational and experimental analysis of redundancy in the central metabolism of Geobacter sulfurreducens.

ABSTRACT: Previous model-based analysis of the metabolic network of Geobacter sulfurreducens suggested the existence of several redundant pathways. Here, we identified eight sets of redundant pathways that included redundancy for the assimilation of acetate, and for the conversion of pyruvate into acetyl-CoA. These equivalent pathways and two other sub-optimal pathways were studied using 5 single-gene deletion mutants in those pathways for the evaluation of the predictive capacity of the model. The growth phenotypes of these mutants were studied under 12 different conditions of electron donor and acceptor availability. The comparison of the model predictions with the resulting experimental phenotypes indicated that pyruvate ferredoxin oxidoreductase is the only activity able to convert pyruvate into

SUBMITTER: Segura D 

PROVIDER: S-EPMC2233667 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature