Openings of the rat recombinant alpha 1 homomeric glycine receptor as a function of the number of agonist molecules bound.

ABSTRACT: The functional properties of rat homomeric alpha 1 glycine receptors were investigated using whole-cell and outside-out recording from human embryonic kidney cells transfected with rat alpha1 subunit cDNA. Whole-cell dose-response curves gave EC(50) estimates between 30 and 120 microM and a Hill slope of approximately 3.3. Single channel recordings were obtained by steady-state application of glycine (0.3, 1, or 10 microM) to outside-out patches. Single channel conductances were mostly 60-90 pS, but smaller conductances of approximately 40 pS were also seen (10% of the events) with a relative frequency that did not depend on agonist concentration. The time constants of the apparent open time distributions did not vary with agonist concentration, but short events were more frequent at low g

SUBMITTER: Beato M 

PROVIDER: S-EPMC2233816 | biostudies-literature | 2002 May

REPOSITORIES: biostudies-literature