Long-term stability of RNA in post-mortem bovine skeletal muscle, liver and subcutaneous adipose tissues.

ABSTRACT:

Background

Recovering high quality intact RNA from post-mortem tissue is of major concern for gene expression studies in animals and humans. Since the availability of post-mortem tissue is often associated with substantial delay, it is important that we understand the temporal variation in the stability of total RNA and of individual gene transcripts so as to be able to appropriately interpret the data generated from such studies. Hence, the objective of this experiment was to qualitatively and quantitatively assess the integrity of total and messenger RNA extracted from bovine skeletal muscle, subcutaneous adipose tissue and liver stored at 4 degrees C at a range of time points up to 22 days post-mortem. These conditions were designed to mimic the environment prevailing during the

SUBMITTER: Bahar B 

PROVIDER: S-EPMC2234424 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature