Characterization of Smoc-1 uncovers two transcript variants showing differential tissue and age specific expression in Bubalus bubalis.

ABSTRACT:

Background

Secreted modular calcium binding protein-1 (Smoc-1) belongs to the BM-40 family which has been implicated with tissue remodeling, angiogenesis and bone mineralization. Besides its anticipated role in embryogenesis, Smoc-1 has been characterized only in a few mammalian species. We made use of the consensus sequence (5' CACCTCTCCACCTGCC 3') of 33.15 repeat loci to explore the buffalo transcriptome and uncovered the Smoc-1 transcript tagged with this repeat. The main objective of this study was to gain an insight into its structural and functional organization, and expressional status of Smoc-1 in water buffalo, Bubalus bubalis.

Results

We cloned and characterized the buffalo Smoc-1, including its copy number status, in-vitro protein expression, tissue & age specific

SUBMITTER: Srivastava J 

PROVIDER: S-EPMC2235864 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature