Development of a species-specific fur gene-based method for identification of the Burkholderia cepacia complex.

ABSTRACT: Burkholderia is an important bacterial genus with a complex taxonomy that contains species of both ecological and pathogenic importance, including nine closely related species collectively termed the Burkholderia cepacia complex (BCC). Unfortunately, 16S rRNA gene analysis has proven to be not sensitive enough to discriminate between species of the BCC. Alternative species identification strategies such as recA-based PCR followed by restriction fragment length polymorphism analysis, although initially useful, have proven to be inaccurate with the increasing species diversity of the BCC. recA gene sequence analysis is more discriminatory and corroborates other biochemical and polyphasic means of taxonomic differentiation. However, it is limited by the fact that certain BCC species are subdi

SUBMITTER: Lynch KH 

PROVIDER: S-EPMC2238088 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature