Application of a microsphere-based array for rapid identification of Acinetobacter spp. with distinct antimicrobial susceptibilities.

ABSTRACT: Acinetobacter spp. have emerged as important nosocomial and multidrug-resistant pathogens in the last decade. A. calcoaceticus, A. baumannii, Acinetobacter genospecies 3, and Acinetobacter genospecies 13TU are genetically closely related and are referred to as the A. calcoaceticus-A. baumannii complex (ACB complex). Distinct Acinetobacter spp. may be associated with differences in antimicrobial susceptibility, so it is important to identify Acinetobacter spp. at the species level. We developed a microsphere-based array that combines an allele-specific primer extension assay and microsphere hybridization for the identification of Acinetobacter spp. This assay can discriminate the 13 different Acinetobacter spp. in less than 8.5 h, and it has high specificity without causing cross-reactivity

SUBMITTER: Lin YC 

PROVIDER: S-EPMC2238122 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature