Adherent self-renewable human embryonic stem cell-derived neural stem cell line: functional engraftment in experimental stroke model.

ABSTRACT:

Background

Human embryonic stem cells (hESCs) offer a virtually unlimited source of neural cells for structural repair in neurological disorders, such as stroke. Neural cells can be derived from hESCs either by direct enrichment, or by isolating specific growth factor-responsive and expandable populations of human neural stem cells (hNSCs). Studies have indicated that the direct enrichment method generates a heterogeneous population of cells that may contain residual undifferentiated stem cells that could lead to tumor formation in vivo.

Methods/principal findings

We isolated an expandable and homogenous population of hNSCs (named SD56) from hESCs using a defined media supplemented with epidermal growth factor (EGF), basic fibroblast growth factor (bFGF) and leukemia inhibit

SUBMITTER: Daadi MM 

PROVIDER: S-EPMC2238795 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature