Vascular sprout formation entails tissue deformations and VE-cadherin-dependent cell-autonomous motility.

ABSTRACT: Embryonic and fetal vascular sprouts form within constantly expanding tissues. Nevertheless, most biological assays of vascular spouting are conducted in a static mechanical milieu. Here we study embryonic mouse allantoides, which normally give raise to an umbilical artery and vein. However, when placed in culture, allantoides assemble a primary vascular network. Unlike other in vitro assays, allantoic primordial vascular cells are situated on the upper surface of a cellular layer that is engaged in robust spreading motion. Time-lapse imaging allows quantification of primordial vascular cell motility as well as the underlying mesothelial tissue motion. Specifically, we calculate endothelial cell-autonomous motion by subtracting the tissue-level mesothelial motion from the total endothelial

SUBMITTER: Perryn ED 

PROVIDER: S-EPMC2239237 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature