Improved functional expression of recombinant human ether-a-go-go (hERG) K+ channels by cultivation at reduced temperature.

ABSTRACT:

Background

HERG potassium channel blockade is the major cause for drug-induced long QT syndrome, which sometimes cause cardiac disrhythmias and sudden death. There is a strong interest in the pharmaceutical industry to develop high quality medium to high-throughput assays for detecting compounds with potential cardiac liability at the earliest stages of drug development. Cultivation of cells at lower temperature has been used to improve the folding and membrane localization of trafficking defective hERG mutant proteins. The objective of this study was to investigate the effect of lower temperature maintenance on wild type hERG expression and assay performance.

Results

Wild type hERG was stably expressed in CHO-K1 cells, with the majority of channel protein being located in t

SUBMITTER: Chen MX 

PROVIDER: S-EPMC2241608 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature