Global transcriptional response of Escherichia coli O157:H7 to growth transitions in glucose minimal medium.

ABSTRACT:

Background

Global patterns of gene expression of Escherichia coli K-12 during growth transitions have been deeply investigated, however, comparable studies of E. coli O157:H7 have not been explored, particularly with respect to factors regulating virulence genes and genomic islands specific to this pathogen. To examine the impact of growth phase on the dynamics of the transcriptome, O157:H7 Sakai strain was cultured in MOPS minimal media (0.1% glucose), RNA harvested at 10 time points from early exponential to full stationary phase, and relative gene expression was measured by co-hybridization on high-density DNA microarrays. Expression levels of 14 genes, including those encoding Shiga toxins and other virulence factors associated with the locus of enterocyte effacement (LEE), wer

SUBMITTER: Bergholz TM 

PROVIDER: S-EPMC2241611 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature