Identifications of conserved 7-mers in 3'-UTRs and microRNAs in Drosophila.

ABSTRACT:

Background

MicroRNAs (miRNAs) are a class of endogenous regulatory small RNAs which play an important role in posttranscriptional regulations by targeting mRNAs for cleavage or translational repression. The base-pairing between the 5'-end of miRNA and the target mRNA 3'-UTRs is essential for the miRNA:mRNA recognition. Recent studies show that many seed matches in 3'-UTRs, which are fully complementary to miRNA 5'-ends, are highly conserved. Based on these features, a two-stage strategy can be implemented to achieve the de novo identification of miRNAs by requiring the complete base-pairing between the 5'-end of miRNA candidates and the potential seed matches in 3'-UTRs.

Results

We presented a new method, which combined multiple pairwise conservation information, to identify

SUBMITTER: Gu J 

PROVIDER: S-EPMC2241842 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature