MicroChIP--a rapid micro chromatin immunoprecipitation assay for small cell samples and biopsies.

ABSTRACT: Chromatin immunoprecipitation (ChIP) is a powerful technique for studying protein-DNA interactions. Drawbacks of current ChIP assays however are a requirement for large cell numbers, which limits applicability of ChIP to rare cell samples, and/or lengthy procedures with limited applications. There are to date no protocols for fast and parallel ChIPs of post-translationally modified histones from small cell numbers or biopsies, and importantly, no protocol allowing for investigations of transcription factor binding in small cell numbers. We report here the development of a micro (micro) ChIP assay suitable for up to nine parallel quantitative ChIPs of modified histones or RNA polymerase II from a single batch of 1000 cells. MicroChIP can also be downscaled to monitor the association of one

SUBMITTER: Dahl JA 

PROVIDER: S-EPMC2241883 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature