Determination of network of residues that regulate allostery in protein families using sequence analysis.

ABSTRACT: Allosteric interactions between residues that are spatially apart and well separated in sequence are important in the function of multimeric proteins as well as single-domain proteins. This observation suggests that, among the residues that are involved in long-range communications, mutation at one site should affect interactions at a distant site. By adopting a sequence-based approach, we present an automated approach that uses a generalization of the familiar sequence entropy in conjunction with a coupled two-way clustering algorithm, to predict the network of interactions that trigger allosteric interactions in proteins. We use the method to identify the subset of dynamically important residues in three families, namely, the small PDZ family, G protein-coupled receptors (GPCR), and the

SUBMITTER: Dima RI 

PROVIDER: S-EPMC2242462 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature