Manipulating and understanding antibiotic production in Streptomyces coelicolor A3(2) with decoy oligonucleotides.

ABSTRACT: We have adapted and extended the decoy oligonucleotide technique for use in prokaryotes. To identify cis-acting regulatory elements within a promoter, we developed a DNase I/T7 exonuclease footprinting technique and applied it to actII-orf4 from Streptomyces coelicolor A3(2), which encodes the pathway-specific activator for production of the antibiotic actinorhodin. Our in vivo mapping data allowed us to create decoy oligonucleotides incorporating the identified regulatory elements and to test whether their introduction into S. coelicolor affected antibiotic production. We mapped the promoter region when in a transcriptionally inactive state before the onset of actinorhodin production with the aim of designing decoy oligonucleotides capable of interfering with potential repressor binding a

SUBMITTER: McArthur M 

PROVIDER: S-EPMC2242687 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature