Genome-wide transcriptional analysis of the human cell cycle identifies genes differentially regulated in normal and cancer cells.

ABSTRACT: Characterization of the transcriptional regulatory network of the normal cell cycle is essential for understanding the perturbations that lead to cancer. However, the complete set of cycling genes in primary cells has not yet been identified. Here, we report the results of genome-wide expression profiling experiments on synchronized primary human foreskin fibroblasts across the cell cycle. Using a combined experimental and computational approach to deconvolve measured expression values into "single-cell" expression profiles, we were able to overcome the limitations inherent in synchronizing nontransformed mammalian cells. This allowed us to identify 480 periodically expressed genes in primary human foreskin fibroblasts. Analysis of the reconstructed primary cell profiles and comparison wit

SUBMITTER: Bar-Joseph Z 

PROVIDER: S-EPMC2242708 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature