Expressed Sequence Tags from the oomycete Plasmopara halstedii, an obligate parasite of the sunflower.

ABSTRACT:

Background

Sunflower downy mildew is a major disease caused by the obligatory biotrophic oomycete Plasmopara halstedii. Little is known about the molecular mechanisms underlying its pathogenicity. In this study we used a genomics approach to gain a first insight into the transcriptome of P. halstedii.

Results

To identify genes from the obligatory biotrophic oomycete Plasmopara halstedii that are expressed during infection in sunflower (Helianthus annuus L.) we employed the suppression subtraction hybridization (SSH) method from sunflower seedlings infected by P. halstedii. Using this method and random sequencing of clones, a total of 602 expressed sequence tags (ESTs) corresponding to 230 unique sequence sets were identified. To determine the origin of the unisequences, PCR

SUBMITTER: Bouzidi MF 

PROVIDER: S-EPMC2242796 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature