Structure of anti-FLAG M2 Fab domain and its use in the stabilization of engineered membrane proteins.

ABSTRACT: The inherent difficulties of stabilizing detergent-solubilized integral membrane proteins for biophysical or structural analysis demand the development of new methodologies to improve success rates. One proven strategy is the use of antibody fragments to increase the ;soluble' portion of any membrane protein, but this approach is limited by the difficulties and expense associated with producing monoclonal antibodies to an appropriate exposed epitope on the target protein. Here, the stabilization of a detergent-solubilized K(+) channel protein, KvPae, by engineering a FLAG-binding epitope into a known loop region of the protein and creating a complex with Fab fragments from commercially available anti-FLAG M2 monoclonal antibodies is reported. Although well diffracting crystals of the compl

SUBMITTER: Roosild TP 

PROVIDER: S-EPMC2242885 | biostudies-literature | 2006 Sep

REPOSITORIES: biostudies-literature