Ontology highlight
ABSTRACT:
SUBMITTER: Tiller T
PROVIDER: S-EPMC2243222 | biostudies-literature | 2008 Jan
REPOSITORIES: biostudies-literature
Tiller Thomas T Meffre Eric E Yurasov Sergey S Tsuiji Makoto M Nussenzweig Michel C MC Wardemann Hedda H
Journal of immunological methods 20071031 1-2
We have developed an efficient strategy that combines immunoglobulin (Ig) gene repertoire analysis and Ig reactivity profiling at the single cell level. Based on surface marker expression individual cells at different stages of human B cell development are isolated by fluorescence-activated cell sorting. For each cell Ig heavy and corresponding Ig light chain gene transcripts are amplified by nested RT-PCR and cloned into eukaryotic expression vectors to produce monoclonal human antibodies of th ...[more]