Expression of genes for bone morphogenetic proteins BMP-2, BMP-4 and BMP-6 in various parts of the human skeleton.

ABSTRACT:

Background

Differences in duration of bone healing in various parts of the human skeleton are common experience for orthopaedic surgeons. The reason for these differences is not obvious and not clear.

Methods

In this paper we decided to measure by the use of real-time RT-PCR technique the level of expression of genes for some isoforms of bone morphogenetic proteins (BMPs), whose role is proven in bone formation, bone induction and bone turnover. Seven bone samples recovered from various parts of skeletons from six cadavers of young healthy men who died in traffic accidents were collected. Activity of genes for BMP-2, -4 and -6 was measured by the use of fluorescent SYBR Green I.

Results

It was found that expression of m-RNA for BMP-2 and BMP-4 is higher in trabecular

SUBMITTER: Kochanowska I 

PROVIDER: S-EPMC2244626 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature