Bacterial flora-typing with targeted, chip-based Pyrosequencing.

ABSTRACT:

Background

The metagenomic analysis of microbial communities holds the potential to improve our understanding of the role of microbes in clinical conditions. Recent, dramatic improvements in DNA sequencing throughput and cost will enable such analyses on individuals. However, such advances in throughput generally come at the cost of shorter read-lengths, limiting the discriminatory power of each read. In particular, classifying the microbial content of samples by sequencing the < 1,600 bp 16S rRNA gene will be affected by such limitations.

Results

We describe a method for identifying the phylogenetic content of bacterial samples using high-throughput Pyrosequencing targeted at the 16S rRNA gene. Our analysis is adapted to the shorter read-lengths of such technology and uses

SUBMITTER: Sundquist A 

PROVIDER: S-EPMC2244631 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature