High throughput gene expression measurement with real time PCR in a microfluidic dynamic array.

ABSTRACT: We describe a high throughput gene expression platform based on microfluidic dynamic arrays. This system allows 2,304 simultaneous real time PCR gene expression measurements in a single chip, while requiring less pipetting than is required to set up a 96 well plate. We show that one can measure the expression of 45 different genes in 18 tissues with replicates in a single chip. The data have excellent concordance with conventional real time PCR and the microfluidic dynamic arrays show better reproducibility than commercial DNA microarrays.

SUBMITTER: Spurgeon SL 

PROVIDER: S-EPMC2244704 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature