Identification of chromosomal alpha-proteobacterial small RNAs by comparative genome analysis and detection in Sinorhizobium meliloti strain 1021.

ABSTRACT:

Background

Small untranslated RNAs (sRNAs) seem to be far more abundant than previously believed. The number of sRNAs confirmed in E. coli through various approaches is above 70, with several hundred more sRNA candidate genes under biological validation. Although the total number of sRNAs in any one species is still unclear, their importance in cellular processes has been established. However, unlike protein genes, no simple feature enables the prediction of the location of the corresponding sequences in genomes. Several approaches, of variable usefulness, to identify genomic sequences encoding sRNA have been described in recent years.

Results

We used a combination of in silico comparative genomics and microarray-based transcriptional profiling. This approach to screening id

SUBMITTER: Ulve VM 

PROVIDER: S-EPMC2245857 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature