Promoter selectivity of the Bacillus subtilis response regulator DegU, a positive regulator of the fla/che operon and sacB.

ABSTRACT:

Background

The response regulator DegU and its cognate histidine kinase DegS constitute a two-component system in the Gram-positive soil bacterium Bacillus subtilis. Unphosphorylated and phosphorylated forms of DegU are known to activate target gene transcription in B. subtilis. Although phosphorylated DegU (DegU-P) regulates more than one hundred and twenty genes, the targets of unphosphorylated DegU are unknown, except for comK.

Results

We found that the fla/che (flagella and chemotaxis) operon is positively regulated by unphosphorylated DegU. The effect was most prominent in a strain bearing the functional swrAA gene, a positive regulator of fla/che. Unphosphorylated DegU bound to two regions in the fla/che regulatory region containing an inverted repeat-like sequence tha

SUBMITTER: Tsukahara K 

PROVIDER: S-EPMC2245950 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature