Fitness of isogenic colony morphology variants of Pseudomonas aeruginosa in murine airway infection.

ABSTRACT: Chronic lung infections with Pseudomonas aeruginosa are associated with the diversification of the persisting clone into niche specialists and morphotypes, a phenomenon called 'dissociative behaviour'. To explore the potential of P. aeruginosa to change its morphotype by single step loss-of-function mutagenesis, a signature-tagged mini-Tn5 plasposon library of the cystic fibrosis airway isolate TBCF10839 was screened for colony morphology variants under nine different conditions in vitro. Transposon insertion into 1% of the genome changed colony morphology into eight discernable morphotypes. Half of the 55 targets encode features of primary or secondary metabolism whereby quinolone production was frequently affected. In the other half the transposon had inserted into genes of the functiona

SUBMITTER: Rakhimova E 

PROVIDER: S-EPMC2246019 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature