Characterization of probiotic Escherichia coli isolates with a novel pan-genome microarray.

ABSTRACT:

Background

Microarrays have recently emerged as a novel procedure to evaluate the genetic content of bacterial species. So far, microarrays have mostly covered single or few strains from the same species. However, with cheaper high-throughput sequencing techniques emerging, multiple strains of the same species are rapidly becoming available, allowing for the definition and characterization of a whole species as a population of genomes--the 'pan-genome'.

Results

Using 32 Escherichia coli and Shigella genome sequences we estimate the pan- and core genome of the species. We designed a high-density microarray in order to provide a tool for characterization of the E. coli pan-genome. Technical performance of this pan-genome microarray based on control strain samples (E. coli K-12

SUBMITTER: Willenbrock H 

PROVIDER: S-EPMC2246269 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature