Transcriptional profiling reveals barcode-like toxicogenomic responses in the zebrafish embryo.

ABSTRACT:

Background

Early life stages are generally most sensitive to toxic effects. Our knowledge on the action of manmade chemicals on the developing vertebrate embryo is, however, rather limited. We addressed the toxicogenomic response of the zebrafish embryo in a systematic manner by asking whether distinct chemicals would induce specific transcriptional profiles.

Results

We exposed zebrafish embryos to a range of environmental toxicants and measured the changes in gene-expression profiles by hybridizing cDNA to an oligonucleotide microarray. Several hundred genes responded significantly to at least one of the 11 toxicants tested. We obtained specific expression profiles for each of the chemicals and could predict the identity of the toxicant from the expression profiles with hig

SUBMITTER: Yang L 

PROVIDER: S-EPMC2246301 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature